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Eleven-year-old Johnny Test lives with his brilliant twin sisters and their eccentric parents. Constantly subjected to his sisters' experiments, Johnny, along with his talking dog Dukey and with the help of allies such as the General and federal agents Mr. Black and Mr. White, embarks on wild adventures filled with mischief and mayhem. Johnny faces school bullies and covert missions, outsmarting adversaries and navigating challenges, all while dodging his sisters' inventions gone awry.