They Drive by Night (1940)

Brothers Paul and Joe Fabrini run a trucking business in California mainly shipping fruit from farms to the markets in Los Angeles. They struggle to make ends meet in the face of corrupt businessmen and intense competition. They are forced into driving long hours, and one night pick up waitress Cassie Hartley who's just quit her job at a truck stop. The three of them witness the death of a mutual acquaintance when he falls asleep at the wheel. This has a profound effect on Paul and Joe and they become determined to find a way to make the business pay so they can quit.